endo-1,4-β-Galactanase (Aspergillus niger

High purity endo-1,4-β-Galactanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.89
CAZy Family: GH53
CAS: 58182-40-4

arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

From Aspergillus niger.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,300 U/mL.

Specific activity:
> 150 U/mg (40oC, pH 4.0 on potato galactan).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-EGALN
1,000 Units
$199.00

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DESCRIPTION

endo-1,4-β-Galactanase (Aspergillus niger)

EC 3.2.1.89
CAZy Family: GH53
CAS: 58182-40-4

Synonyms
arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 4 years at 4oC.

Specific activity:
~ 400 U/mg (40oC, pH 4.0 on potato galactan).

Unit definition:
One Unit of galactanase activity is defined as the amount of enzyme required to release one µmole of galactose reducing-sugar equivalents per minute from potato galactan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40oC.

Specificity:
endo-hydrolysis of (1,4)-β-D-galactose linkages in (1,4)-β-galactans and type I arabinogalactans.

Monoclonal antibody-based analysis of cell wall remodeling during xylogenesis.

Shinohara, N., Kakegawa, K. & Fukuda, H. (2015). Journal of Plant Research, 128(6), 975-986.

Digestibility and prebiotic properties of potato rhamnogalacturonan I polysaccharide and its galactose-rich oligosaccharides/oligomers.

Khodaei, N., Fernandez, B., Fliss, I. & Karboune, S. (2016). Carbohydrate Polymers, 136, 1074-1084.

Remodeling of pectin and hemicelluloses in tomato pericarp during fruit growth.

Guillon, F., Moïse, A., Quemener, B., Bouchet, B., Devaux, M. F., Alvarado, C. & Lahaye, M. (2017). Plant Science, 257, 48-62.

Characterization of Cell Wall Composition of Radish (Raphanus sativus L. var. sativus) and Maturation Related Changes.

Schäfer, J., Brett, A., Trierweiler, B. & Bunzel, M. (2016). Journal of Agricultural and Food Chemistry, 64(45), 8625-8632.