endo-1,4-β-Galactanase (Clostridium thermocellum

High purity recombinant endo-1,4-β-Galactanase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.89
CAZy Family: GH53
CAS: 58182-40-4 

arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

Recombinant. From Clostridium thermocellum.
In 3.2 M ammonium sulphate.
Supplied at ~ 125 U/mL. 

Specific activity:
~ 15 U/mg (40oC, pH 4.5 on potato galactan);
~ 30 U/mg (60oC, pH 4.5 on potato galactan).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-GALCT
350 Units at 40oC; ~ 770 Units at 60oC
$216.00

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DESCRIPTION

endo-1,4-β-Galactanase (Clostridium thermocellum)

EC 3.2.1.89
CAZy Family: GH53
CAS: 58182-40-4

Synonyms:
arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 15 U/mg (40oC, pH 4.5 on potato galactan);
~ 30 U/mg (60oC, pH 4.5 on potato galactan).

Unit definition:
One Unit of galactanase activity is defined as the amount of enzyme required to release one μmole of galactose reducing-sugar equivalents per minute from potato galactan in sodium acetate buffer (100 mM), pH 4.5.

Specificity:
endo-hydrolysis of (1,4)-β-D-galactosidic linkages in galactans.

Applications:
Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.

Structural modifications of sugar beet pectin and the relationship of structure to functionality.

Funami, T., Nakauma, M., Ishihara, S., Tanaka, R., Inoue, T. & Phillips, G. O. (2011). Food Hydrocolloids, 25(2), 221-229.