endo-1,4-β-Xylanase (Bacillus stearothermophilus T6) 

High purity recombinant endo-1,4-β-Xylanase (Bacillus stearothermophilus T6) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Recombinant. From Bacillus stearothermophilus T6.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,000 U/mL. 

Specific activity:
~ 65 U/mg (70oC, pH 6.5 on wheat arabinoxylan);
~ 12 U/mg (40oC, pH 6.5 on wheat arabinoxylan).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-XYNBS
2,000 Units at 70oC
$185.00

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DESCRIPTION

endo-1,4-β-Xylanase (Bacillus stearothermophilus T6)

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

Synonyms:
endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 65 U/mg (70oC, pH 6.5 on wheat arabinoxylan);
~ 12 U/mg (40oC, pH 6.5 on wheat arabinoxylan).

Unit definition:
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in MES buffer (100 mM), pH 6.5.

Specificity:
endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries.

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.

Extraction of soluble arabinoxylan from enzymatically pretreated wheat bran and production of short xylo-oligosaccharides and arabinoxylo-oligosaccharides from arabinoxylan by glycoside hydrolase family 10 and 11 endoxylanases.

Mathew, S., Karlsson, E. N. & Adlercreutz, P. (2017). Journal of Biotechnology, 260, 53-61.