endo-1,4-β-Xylanase (Cellvibrio japonicus

High purity recombinant endo-1,4-β-Xylanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Recombinant. Catalytic domain from Cellvibrio japonicus.
In 3.2 M ammonium sulphate.
Supplied at ~ 500 U/mL. 

Specific activity:
~ 38 U/mg (40oC, pH 5.0 on Wheat arabinoxylan);
~ 62 U/mg (60oC, pH 5.0 on wheat arabinoxylan).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-XYNACJ
500 Units at 40oC
$201.00

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DESCRIPTION

endo-1,4-β-Xylanase (Cellvibrio japonicus)

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

Synonyms:
endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 38 U/mg (40oC, pH 5.0 on Wheat arabinoxylan); 
~ 62 U/mg (60oC, pH 5.0 on wheat arabinoxylan).

Unit definition:
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0.

Specificity:
endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries. 

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.