endo-1,4-β-Xylanase (Cellvibrio mixtus

High purity recombinant endo-1,4-β-Xylanase (Cellvibrio mixtus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Recombinant. Catalytic domain of Xyn10B from Cellvibrio mixtus.
In 3.2 M ammonium sulphate.
Supplied at ~ 750 U/mL. 

Specific activity:
~ 15 U/mg (40oC, pH 6.5 on wheat arabinoxylan);
~ 30 U/mg (50oC, pH 6.5 on wheat arabinoxylan).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-XYNBCM
1,500 Units
$216.00

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DESCRIPTION

endo-1,4-β-Xylanase (Cellvibrio mixtus)

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

Synonyms:
endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 15 U/mg (40oC, pH 6.5 on wheat arabinoxylan); 
~ 30 U/mg (50oC, pH 6.5 on wheat arabinoxylan).

Unit definition:
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in sodium phosphate buffer (100 mM), pH 6.5.

Specificity:
endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries.

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

A Comparison of Polysaccharide Substrates and Reducing Sugar Methods for the Measurement of endo-1,4-β-Xylanase

McCleary, B. V. & McGeough, P. (2015). Appl. Biochem. Biotechnol., 177(5), 1152-1163.

Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp.

Phan, J. L., Tucker, M. R., Khor, S. F., Shirley, N., Lahnstein, J., Beahan, C., A., Bacic. & Burton, R. A. (2016). Journal of Experimental Botany, 67(22), 6481-6495.