endo-1,4-β-Xylanase M3 (Trichoderma longibrachiatum

High purity endo-1,4-β-Xylanase M3 (Trichoderma longibrachiatum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8
CAZy Family: GH11
CAS: 9025-57-4 

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Highly purified. From Trichoderma longibrachiatum. Electrophoretically homogeneous, pI 9.0.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,600 U/mL. 

Specific activity:
> 100 U/mg (40oC, pH 6.0 on wheat arabinoxylan).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-XYTR3
8,000 Units
$199.00

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DESCRIPTION

endo-1,4-β-Xylanase M3 (Trichoderma longibrachiatum)

EC 3.2.1.8
CAZy Family: GH11

CAS: 9025-57-4

Synonyms:
endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
> 100 U/mg (40oC, pH 6.0 on wheat arabinoxylan).

Unit definition:
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (10 mg/mL) in sodium phosphate buffer (100 mM), pH 6.0 at 40oC. 

Specificity:
endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

Evaluation of the xylan breakdown potential of eight mesophilic endoxylanases.

Cuyvers, S., Dornez, E., Moers, K., Pollet, A., Delcour, J. A. & Courtin, C. M. (2011). Enzyme and Microbial Technology, 49(3), 305-311.

His374 of wheat endoxylanase inhibitor TAXI‐I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases.

Fierens, K., Gils, A., Sansen, S., Brijs, K., Courtin, C. M., Declerck, P. J., De Ranter, C. J., Gebruers, K., Rabijns, A., Robben, J., Van Campenhout, S., Volckaert, G. & Delcour, J. A. (2005). FEBS Journal, 272(22), 5872-5882.

Induction of a novel XIP-type xylanase inhibitor by external ascorbic acid treatment and differential expression of XIP-family genes in rice.

Tokunaga, T. & Esaka, M. (2007). Plant and Cell Physiology, 48(5), 700-714.

ESEM Study of the Effects of Hydrolytic Enzymes on Wheat Bran Structure.

Douge, M., Nonus, M., Thomasset, T., Teissier, P. & Barbeau, J. Y. (2004). Microscopy and Analysis, 18(6), 21-24.