endo-1,4-β-Xylanase (rumen microorganism)

High purity endo-1,4-β-Xylanase (rumen microorganism) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8
CAZy Family: GH11
CAS: 9025-57-4 

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Highly purified. From rumen microorganism. Single band on SDS-gel electrophoresis (MW 21,000).
In 3.2 M ammonium sulphate.
Supplied at ~ 2,100 U/mL. 

Specific activity:
~ 380 U/mg (40oC, pH 6.0 on wheat arabinoxylan).

Stability:  > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-XYRU6
8,000 Units
$199.00

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DESCRIPTION

endo-1,4-β-Xylanase (rumen microorganism)

EC 3.2.1.8
CAZy Family: GH11

CAS: 9025-57-4

Synonyms:
endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 380 U/mg (40oC, pH 6.0 on wheat arabinoxylan).

Unit definition:
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (10 mg/mL) in sodium phosphate buffer (100 mM), pH 6.0 at 40oC.

Specificity:
endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries.

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Identification of quantitative trait loci affecting hemicellulose characteristics based on cell wall composition in a wild and cultivated rice species.

Zhang, S. J., Song, X. Q., Yu, B. S., Zhang, B. C., Sun, C. Q., Knox, J. P. & Zhou, Y. H. (2012). Molecular Plant, 5(1), 162-175.

The use of Xylanases from different microbial origin in bread baking and their effects on bread qualities.

Al-Widyan, O., Khataibeh, M. H. & Abu-Alruz, K. (2008). Journal of Applied Sciences, 8(4), 672-676.

Emergence of a subfamily of xylanase inhibitors within glycoside hydrolase family 18.

Durand, A., Hughes, R., Roussel, A., Flatman, R., Henrissat, B. & Juge, N. (2005). FEBS Journal, 272(7), 1745-1755.

Analysis of the arabinoxylan arabinofuranohydrolase gene family in barley does not support their involvement in the remodelling of endosperm cell walls during development.

Laidlaw, H. K. C., Lahnstein, J., Burton, R. A., Fincher, G. B. & Jobling, S. A. (2012). Journal of Experimental Botany, 63(8), 3031-3045.

Arabidopsis and Brachypodium distachyon transgenic plants expressing Aspergillus nidulans acetylesterases have decreased degree of polysaccharide acetylation and increased resistance to pathogens.

Pogorelko, G., Lionetti, V., Fursova, O., Sundaram, R. M., Qi, M., Whitham, S. A., Bogdanove, A. J., Bellincampi, D. & Zabotina, O. A. (2013). Plant Physiology, 162(1), 9-23.

Regulation of the cellulose synthase-like gene family by light in the maize mesocotyl.

Van Erp, H. & Walton, J. D. (2009). Planta, 229(4), 885-897.

Biochemical characterization of a halotolerant feruloyl esterase from Actinomyces spp.: refolding and activity following thermal deactivation.

Hunt, C. J., Tanksale, A. & Haritos, V. S. (2016). Applied Microbiology and Biotechnology, 100(4), 1777-1787.

Regiospecific acetylation of xylan is mediated by a group of DUF231-containing O-acetyltransferases.

Zhong, R., Cui, D. & Ye, Z. H. (2017). Plant and Cell Physiology, In Press.