endo-1,4-β-Xylanase (Thermotoga maritima

High purity recombinant endo-1,4-β-Xylanase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Recombinant. Catalytic domain of Xyn10A from Thermotoga maritima.
In 3.2 M ammonium sulphate.
Supplied at ~ 3,750 U/mL. 

Specific activity:
~ 115 U/mg (80oC, pH 5.0 on wheat arabinoxylan);
~ 22 U/mg (40oC, pH 5.0 on wheat arabinoxylan).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-XYLATM
7,500 Units at 80oC
$201.00

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DESCRIPTION

endo-1,4-β-Xylanase (Thermotoga maritima)

EC 3.2.1.8
CAZy Family: GH10
CAS: 9025-57-4

Synonyms:
endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 115 U/mg (80oC, pH 5.0 on wheat arabinoxylan); 
~ 22 U/mg (40oC, pH 5.0 on wheat arabinoxylan).

Unit definition:
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0.

Specificity:
endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries. 

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

The Multi Domain Caldicellulosiruptor bescii CelA Cellulase Excels at the Hydrolysis of Crystalline Cellulose.

Brunecky, R., Donohoe, B. S., Yarbrough, J. M., Mittal, A., Scott, B. R., Ding, H., Taylor II, L., E., Russell, J. F., Chung, D., Westpheling, J., Teter, S. A., Himmel, M. E. & Bomble, Y. J. (2017). Scientific Reports, 7, 9622.