endo-Polygalacturonanase M2 (Aspergillus aculeatus

High purity endo-Polygalacturonanase M2 (Aspergillus aculeatus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.15
CAZy Family: GH28
CAS: 9032-75-1

polygalacturonase; (1->4)-alpha-D-galacturonan glycanohydrolase

Highly purified. From Aspergillus aculeatus.
In 3.2 M ammonium sulphate.
Supplied at ~ 6,500 U/mL. 

Specific Activity:
~ 300 U/mg (40oC, pH 5.5 on polygalacturonic acid).

Stability: > 4 years at 4oC.

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Product Code
Content/size
Stock
Price
Qty
E-PGALUSP
10,000 Units
 

DESCRIPTION

endo-Polygalacturonanase M2 (Aspergillus aculeatus)

EC 3.2.1.15
CAZy Family: GH28
CAS: 9032-75-1

Synonyms:
polygalacturonase; (1->4)-alpha-D-galacturonan glycanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 300 U/mg (40oC, pH 5.5 on polygalacturonic acid).

Unit definition:
One Unit of endo-polygalacturonanase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (10 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40oC.

Specificity:
Random hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans.

Applications:
Applications in carbohydrate research and in the food industry.

Enhanced electrostatic interactions in tomato cell suspensions.

Sankaran, A. K., Nijsse, J., Bialek, L., Shpigelman, A., Hendrickx, M. E. & Van Loey, A. M. (2015). Food Hydrocolloids, 43, 442-450.