endo-Polygalacturonanase M2 (Aspergillus aculeatus

High purity endo-Polygalacturonanase M2 (Aspergillus aculeatus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: GH28
CAS: 9032-75-1

polygalacturonase; (1->4)-alpha-D-galacturonan glycanohydrolase

Highly purified. From Aspergillus aculeatus.
In 3.2 M ammonium sulphate.
Supplied at ~ 650 U/mL. 

Specific Activity:
~ 150 U/mg (40oC, pH 5.5 on polygalacturonic acid).

Stability: > 4 years at 4oC.

Product Code
5,000 Units

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endo-Polygalacturonanase M2 (Aspergillus aculeatus)

CAZy Family: GH28
CAS: 9032-75-1

polygalacturonase; (1->4)-alpha-D-galacturonan glycanohydrolase

In 3.2 M ammonium sulphate.

> 4 years at 4oC.

Specific activity:
~ 150 U/mg (40oC, pH 5.5 on polygalacturonic acid).

Unit definition:
One Unit of endo-polygalacturonanase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40oC.

Random hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans.

Applications in carbohydrate research and in the food industry.

Enhanced electrostatic interactions in tomato cell suspensions.

Sankaran, A. K., Nijsse, J., Bialek, L., Shpigelman, A., Hendrickx, M. E. & Van Loey, A. M. (2015). Food Hydrocolloids, 43, 442-450.

Cell separation in kiwifruit without development of a specialised detachment zone.

Prakash, R., Hallett, I. C., Wong, S. F., Johnston, S. L., O’Donoghue, E. M., McAtee, P. A., Seal, A. G., Atkinson, R. G. & Schröder, R. (2017). BMC Plant Biology, 17(1), 86.

Introduction and characterization of charged functional domains into an esterified pectic homogalacturonan by a citrus pectin methylesterase and comparison of its modes of action to other pectin methylesterase isozymes.

Kim, Y., Williams, M. A., Luzio, G. A. & Cameron, R. G. (2017). Food Hydrocolloids, 69, 422-431.

Partial purification of a polygalacturonase from a new Aspergillus sojae mutant and its application in grape mash maceration.

Yıldız, S., Mata‐Gómez, M. A., Tarı, C. & Rito‐Palomares, M. (2017). International Journal of Food Science & Technology, 52(3), 834-842.

Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC.

Wu, D. T., Cheong, K. L., Deng, Y., Lin, P. C., Wei, F., Lv, X. J., Long, Z. R., Zhao, J., Ma, S. C. & Li, S. P. (2015). Carbohydrate polymers, 134, 12-19.

Remodeling of pectin and hemicelluloses in tomato pericarp during fruit growth.

Guillon, F., Moïse, A., Quemener, B., Bouchet, B., Devaux, M. F., Alvarado, C. & Lahaye, M. (2017). Plant Science, 257, 48-62.

Boron bridging of rhamnogalacturonan‐II is promoted in vitro by cationic chaperones, including polyhistidine and wall glycoproteins.

Chormova, D. & Fry, S. C. (2016). New Phytologist, 209(1), 241-251.

Structure characterization, chemical and enzymatic degradation, and chain conformation of an acidic polysaccharide from Lycium barbarum L.

Liu, W., Liu, Y., Zhu, R., Yu, J., Lu, W., Pan, C., Yao, W. & Gao, X. (2016). Carbohydrate Polymers, 147, 114-124.

Macrophages treated with non-digestible polysaccharides reveal a transcriptionally unique phenotype.

Tang, Y., Govers, C., Wichers, H. J. & Mes, J. J. (2017). Journal of Functional Foods, 36, 280-289.

Stomatal opening involves polar, not radial, stiffening of guard cells.

Carter, R., Woolfenden, H., Baillie, A., Amsbury, S., Carroll, S., Healicon, E., Sovatzoglou, S., Braybrook, S., Gray, J. E., Hobbs, J., Morris, R. J. & Morris, R. J. (2017). Current Biology, 27(19), 2974-2983.

Altered lignification in mur1-1 a mutant deficient in GDP-L-fucose synthesis with reduced RG-II cross linking.

Voxeur, A., Soubigou-Taconnat, L., Legée, F., Sakai, K., Antelme, S., Durand-Tardif, M., Lapierre, C. & Sibout, R. (2017). PloS one, 12(9), e0184820.

Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal.

Sinclair, S. A., Larue, C., Bonk, L., Khan, A., Castillo-Michel, H., Stein, R. J., Grolimund, D., Begerow, D., Neumann, U., Haydon, M. J. & Krämer, U. (2017). Current Biology, In Press.

Structural and functional effects of manipulating the degree of methylesterification in a model homogalacturonan with a pseudo-random fungal pectin methylesterase followed by a processive methylesterase.

Kim, Y., Cameron, R. G., Williams, M. A. K. & Luzio, G. A. (2017). Food Hydrocolloids, In Press.

Characterization of xylan in the early stages of secondary cell wall formation in tobacco bright yellow-2 cells.

Ishii, T., Matsuoka, K., Ono, H., Ohnishi-Kameyama, M., Yaoi, K., Nakano, Y., Ohtani, M., Demura, T., wai. H. & Satoh, S. (2017). Carbohydrate Polymers, 176, 381-391.

Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.

Oliva, S. R., Mingorance, M. D., Sanhueza, D., Fry, S. C. & Leidi, E. O. (2018). Plant Physiology and Biochemistry, In Press.

Pectin oligosaccharides from sugar beet pulp: molecular characterization and potential prebiotic activity.

Prandi, B., Baldassarre, S., Babbar, N., Bancalari, E., Vandezande, P., Hermans, D., Bruggeman, G., Gatti, M., Elst, K. & Stefano Sforza, S. (2018). Food & Function, In Press.

Active pectin fragments of high in vitro antiproliferation activities toward human colon adenocarcinoma cells: Rhamnogalacturonan II.

Ai, L., Chung, Y. C., Lin, S. Y., Lee, K. C., Lai, P. F. H., Xia, Y., Wang, G. & Cui, S. W. (2018). Food Hydrocolloids, 83, 239-245.