β-D-Xylosidase (Selenomonas ruminantium

High purity recombinant exo-1,4-β-D-Xylosidase (Selenomonas ruminantium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.37
CAZy Family: GH43
CAS: 9025-53-0

xylan 1,4-beta-xylosidase; 4-beta-D-xylan xylohydrolase

Recombinant. From Selenomonas ruminantium.
In 3.2 M ammonium sulphate.
E-BXSR-1KU: Supplied at ~ 500 U/mL.
E-BXSR-3KU: Supplied at ~ 500 U/mL. 

Specific activity: ~ 115 U/mg (40oC, pH 5.3 on pNP-β-D-xylanopyranoside).

Stability: > 2 years at 4oC.

Data booklets for each pack size are located in the Documentation tab.

Product Code
Content/Size
Stock
Price
Qty
E-BXSR-1KU
1,000 units
$124.00
E-BXSR-3KU
3,000 units
$225.00

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DESCRIPTION

β-D-Xylosidase (Selenomonas ruminantium)

EC 3.2.1.37 
CAZy Family: GH43
CAS: 9025-53-0

Synonyms:
xylan 1,4-beta-xylosidase; 4-beta-D-xylan xylohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 115 U/mg (40oC, pH 5.3 on pNP-β-D-xylanopyranoside).

Unit definition:
One Unit of β-xylosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-xylopyranoside (5 mM) in sodium succinate buffer (50 mM), pH 5.3 at 40oC.

Specificity:
Hydrolysis of (1,4)-β-D-xylans and xylo-oligosaccharides to remove successive D-xylose residues from non-reducing termini.

Applications:
Applications in carbohydrate and biofuels research.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

Formulation of enzyme blends to maximize the hydrolysis of alkaline peroxide pretreated alfalfa hay and barley straw by rumen enzymes and commercial cellulases.

Badhan, A., Wang, Y., Gruninger, R., Patton, D., Powlowski, J., Tsang, A. & McAllister, T. (2014). BMC Biotechnology, 14(1), 31.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.