exo-Polygalacturonase

High purity recombinant exo-polygalacturonase (Yersinia enterocolitica) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.82
CAZy Family: GH28
CAS: 37288-58-7

exo-poly-alpha-galacturonosidase

Recombinant. From Yersinia enterocolitica.
In 3.2 M ammonium sulphate.
Supplied at ~ 900 U/mL.

Specific activity: 
~ 220 U/mg protein (60oC, pH 6.0 on Polygalacturonic Acid).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-EXPGA
500 U at 60oC
$195.00

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DESCRIPTION

exo-Polygalacturonase (Yersinia enterocolitica)

EC 3.2.1.82
CAZy Family: GH28
CAS: 37288-58-7

Synonyms:
exo-poly-alpha-galacturonosidase.

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC. 

Specific activity:
~ 220 U/mg protein (60oC, pH 6.0 on Polygalacturonic Acid).

Unit definition:
One Unit of exo-polygalacturonase activity is defined as the amount of enzyme required to release one μmole of reducing-sugar equivalents per minute from Polygalacturonic Acid (10 mg/mL) in potassium phosphate buffer (100 mM), pH 6.0.

Specificity:
Hydrolysis of pectic acid from the non-reducing end, releasing digalacturonate.

Applications:
For use in research.

The structural basis for exopolygalacturonase activity in a family 28 glycoside hydrolase.

Abbott, D. W. & Boraston, A., B. (2007). Journal of Molecular Biology, 368(5), 1215-1222.