exo-α-Sialidase (Salmonella typhimurium)

High purity recombinant exo-α-sialidase (Salmonella typhimurium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.18
CAZy Family: GH33
CAS: 9001-67-6

exo-alpha-sialidase; acetylneuraminyl hydrolase

Recombinant. From Salmonella typhimurium.
In 3.2 M ammonium sulphate.
Supplied at ~ 2,500 U/mL. 

Specific activity: 
~ 750 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid).

Stability: >2 years at 4oC.

Custom quantities and formulations available up on request. 

Product Code
Content/size
Stock
Price
Qty
E-SIALST
250 Units
$201.00

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DESCRIPTION

exo-α-Sialidase (Salmonella typhimurium)

EC 3.2.1.18
CAZy Family: GH33
CAS: 9001-67-6

Synonyms:
exo-alpha-sialidase; acetylneuraminyl hydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 750 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid).

Unit definition:
One Unit of sialidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37oC.

Specificity:
Hydrolysis of unbranched, non-reducing terminal α-2,3-linked >> α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.

Applications:
Applications in clinical chemistry, glycobiology and carbohydrate research.