myo-Inositol Assay Kit

The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in foods, dairy products and various other materials.

Phytic acid content of samples with very low levels of free myo-inositol can also be determined using K-INOSL. This can be achieved by measuring the amount of myo-inositol released after de-phosphorylation of phytic acid with the enzymes phytase and alkaline phosphatase, as used with the Megazyme Phytic Acid/Total Phosphorus Assay Kit (K-PHYT).

Not suitable for the determination of myo-inositol in baby formula.

Product Code
Content/size
Stock
Price
Qty
K-INOSL
50 assays per kit
$272.00

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Colourimetric method for the determination of myo-Inositol
in various sample matrices

Principle:
                (myo-inositol dehydrogenase)
(1) myo-Inositol + NAD+
                   2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+


                          (diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan

Kit size:    50 assays

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 492 nm
Reaction time:                  ~ 25 min
Detection limit:                 0.8 mg/L
Application examples:
Animal feeds, food, baby milk formulation and other materials
Method recognition:       Novel method

Advantages

  • Very cost effective
     
  • All reagents stable for > 2 years after preparation
     
  • Only enzymatic kit available
     
  • Rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene.

Takeno, S., Hori, K., Ohtani, S., Mimura, A., Mitsuhashi, S. & Ikeda, M. (2016). Metabolic engineering, 37, 1-10.

Regulation of myo-inositol biosynthesis by p53-ISYNA1 pathway.

Koguchi, T., Tanikawa, C., Mori, J., Kojima, Y. & Matsuda, K. (2016). International Journal of Oncology, 48(6), 2415-2424.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs